Professor of Molecular Biology at the School of Medicine, 2nd University of Naples. & Adjunct Professor, Sbarro Inst. for Cancer Research and Mol. Medicine, Temple Univ, Philadelphia, USA.
Invited Talk Title: Myeloma cells can corrupt senescent mesenchymal stromal cells and impair their anti-tumor activity
Senescent cells secrete several molecules that help to prevent the progression of cancer. However, cancer cells can also misuse these secreted elements to survive and grow. Since the molecular and functional bases of these different elements remain poorly understood, we analyzed the effect of senescent mesenchymal stromal cell (MSC) secretome on the biology of ARH-77 myeloma cells. In addition to differentiating in mesodermal derivatives, MSCs have sustained interest among researchers by supporting hematopoiesis, contributing to tissue homeostasis, and modulating inflammatory response, all activities accomplished primarily by the secretion of cytokines and growth factors. Moreover, senescence profoundly affects the composition of MSC secretome. In this study, we induced MSC senescence by oxidative stress, DNA damage, and replicative exhaustion. While the first two are considered to induce acute senescence, extensive proliferation triggers replicative (i.e., chronic) senescence. We cultivated cancer cells in the presence of acute and chronic senescent MSC-conditioned media and evaluated their proliferation, DNA damage, apoptosis, and senescence. Our findings revealed that senescent secretomes induced apoptosis or senescence, if not both, to different extents. This anti-tumor activity became heavily impaired when secretomes were collected from senescent cells previously in contact (i.e., primed) with cancer cells. Our analysis of senescent MSC secretomes with LC-MS/MS followed by Gene Ontology classification further indicated that priming with cancer profoundly affected secretome composition by abrogating the production of pro-senescent and apoptotic factors. We thus showed for the first time that compared with cancer-primed MSCs, naïve senescent MSCs can exert different effects on tumor progression.
Name and Surname: Umberto Galderisi
Place and date of birth: Salerno, September 10 1963
Degree: Master of Science in Biology, PhD in Molecular Biology
Tel : + 39 081 5667585
web site: www.umbertogalderisi.it
Professor of Molecular Biology at the School of Medicine, 2nd University of Naples.
Adjunct Professor, Sbarro Inst. for Cancer Research and Mol. Medicine, Temple Univ, Philadelphia, USA.
National Association of Italian Biologist.
ISSCR (International Society for Stem Cell Researches).
International Society for Cellular Therapy (ISCT).
ECSA (European Cellular Senescence Association).
Founder and President of Stem Cell Research Italy, scientific association of researchers involved in stem cell
1987 Master degree in Biological Sciences, summa cum laude, University of Naples, Naples, Italy.
1992 PhD, Department of Genetics, General and Molecular Biology, University of Naples, Naples, Italy.
Main Work experiences
1996 Researcher at “Museum National d’Histoire Naturelle” in Paris, France.
1997/1999 Researcher at CEINGE (Biotech and Molecular Biology Center), Napoli, Italy.
1998/1999 Visiting Researcher at, Jefferson Medical College, Philadelphia, PA, USA.
2001 to present Adjunct Researcher and Professor at Sbarro Inst, Temple University, Philadelphia, USA.
2003 Visiting Researcher at Center for Gene Theraphy, Tulane Univ, New Orleans, LA, USA.
2006/2011 Visiting Scientist at Heinrich Pette Inst of Hamburg University, Germany.
Member of the following Editorial Boards
World Journal of Stem Cells, World Journal of Experimental Medicine , Stem Cell Discovery, Stem Cell
Review and Reports,, ISRN Stem Cells, Cancer & Clinical Research Journal, Frontier in Stem Cell
Treatment, Journal of Cardiology and Therapy, Archives of Cytology, Imaging Journal of Clinical and Medical
Sciences, Austin Journal of Molecular and Cellular Biology
He is co-author of more than 112 articles (H-index 31)
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